BayGenomics In Situ Hybridization Studies on Trapped Genes

These studies are led by Dr. Pao-Tien Chuang, Associate Professor at the Cardiovascular Research Institute at UCSF. EST clones are obtained corresponding to genes inactivated in BayGenomics gene-trap studies and probes are generated for in situ hybridization studies. When a mouse gene is identified that appears particularly interesting (e.g., a highly restricted pattern of expression in the heart or lung during embryonic development), the pattern of expression of the zebrafish orthologue is defined to assess evolutionary conservation of gene-expression patterns.

Image Clones were purchased from Research Genetics. Each gene is analyzed on two tissues from different developmental stages, either 11.5, 12.5, 13.5 or 18.5 days post coitus (dpc), and on adult lung tissues, that have been treated with either bleomycin or ovalbumin to mimic lung fibrosis and asthma respectively.